Smad4 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-23966R-APC |
| Product Name | Smad4 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Smad4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4089 |
| Swiss Prot | Q13485 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Mothers against decapentaplegic homolog 4; (Small) Mothers Against Decapentaplegic; Deleted in Pancreatic Carcinoma; Deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; DPC 4; DPC4; hSMAD4; JIP; MAD homolog 4; MAD mothers against decapentaplegic Drosophila homolog 4; MAD mothers against decapentaplegic homolog 4; MADH 4; MADH4; Med; Medea; Mothers against decapentaplegic homolog 4; Mothers against DPP homolog 4; OTTHUMP00000163548; Smad 4; SMAD family member 4; SMAD mothers against DPP homolog 4. |
| Background | Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Smad4 Polyclonal Antibody, APC Conjugated (bs-23966R-APC) validated for, and at what dilution?
bs-23966R-APC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Smad4 Polyclonal Antibody, APC Conjugated (bs-23966R-APC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.
How should bs-23966R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Smad4 Polyclonal Antibody, APC Conjugated (bs-23966R-APC)?
KLH conjugated synthetic peptide derived from human Smad4
What are the excitation and emission wavelengths of the APC conjugate (bs-23966R-APC)?
APC on bs-23966R-APC: excitation / emission 650nm/660nm.