Help

CRHR2 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Rabbit
Overview
Catalog # bs-23975R-Cy3
Product Name CRHR2 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CRHR2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1395
Swiss Prot Q13324
Subcellular location Cell membrane
Synonyms Corticotropin releasing hormone receptor 2; Corticotropin-releasing factor receptor 2; Corticotropin-releasing hormone receptor 2; CRF 2; CRF 2R; CRF R2; CRF-R-2; CRF-R2; CRF2; CRF2R; CRFR 2; CRFR-2; CRFR2; CRFR2_HUMAN; CRH 2R; CRH R2; CRH-R-2; CRH-R2; CRH2R; CRHR 2; CRHR2.
Background This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is CRHR2 Polyclonal Antibody, Cy3 Conjugated (bs-23975R-Cy3) validated for, and at what dilution?

bs-23975R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CRHR2 Polyclonal Antibody, Cy3 Conjugated (bs-23975R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Rabbit.

How should bs-23975R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CRHR2 Polyclonal Antibody, Cy3 Conjugated (bs-23975R-Cy3)?

KLH conjugated synthetic peptide derived from human CRHR2

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-23975R-Cy3)?

Cy3 on bs-23975R-Cy3: excitation / emission 512,550nm/570,615nm.