AKR1B1 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2405R-BF555 |
| Product Name | AKR1B1 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may have a secondary cross-reactivity with AKR1C1/2/3/4 due to a 75% sequence similarity in the corresponding immunogen regions. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AKR1B1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 231 |
| Swiss Prot | P15121 |
| Subcellular location | Cytoplasm |
| Synonyms | AR; ADR; ALR2; ALDR1; Aldose reductase; Aldehyde reductase; Aldo-keto reductase family 1 member B1; AKR1B1 |
| Background | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AKR1B1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2405R-BF555) validated for, and at what dilution?
bs-2405R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AKR1B1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2405R-BF555) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Rabbit.
How should bs-2405R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AKR1B1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2405R-BF555)?
KLH conjugated synthetic peptide derived from human AKR1B1
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-2405R-BF555)?
AbBy Fluor-555 on bs-2405R-BF555: excitation / emission 553nm/568nm.