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AKR1B1 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Rabbit
Overview
Catalog # bs-2405R-PerCP-100uL
Product Name AKR1B1 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody may have a secondary cross-reactivity with AKR1C1/2/3/4 due to a 75% sequence similarity in the corresponding immunogen regions.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AKR1B1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 231
Swiss Prot P15121
Subcellular location Cytoplasm
Synonyms AR; ADR; ALR2; ALDR1; Aldose reductase; Aldehyde reductase; Aldo-keto reductase family 1 member B1; AKR1B1
Background Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is AKR1B1 Polyclonal Antibody, PerCP Conjugated (bs-2405R-PerCP) validated for, and at what dilution?

bs-2405R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AKR1B1 Polyclonal Antibody, PerCP Conjugated (bs-2405R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Rabbit.

How should bs-2405R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AKR1B1 Polyclonal Antibody, PerCP Conjugated (bs-2405R-PerCP)?

KLH conjugated synthetic peptide derived from human AKR1B1

What are the excitation and emission wavelengths of the PerCP conjugate (bs-2405R-PerCP)?

PerCP on bs-2405R-PerCP: excitation / emission 482nm/677nm.