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Aldolase A Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Horse
  • Rabbit
Overview
Catalog # bs-2406R-FITC
Product Name Aldolase A Polyclonal Antibody, FITC Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Aldolase A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 226
Swiss Prot P04075
Subcellular location Cytoplasm
Synonyms ALDA; GSD12; HEL-S-87p; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase; ALDOA
Background Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Aldolase A Polyclonal Antibody, FITC Conjugated (bs-2406R-FITC) validated for, and at what dilution?

bs-2406R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Aldolase A Polyclonal Antibody, FITC Conjugated (bs-2406R-FITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Horse, Rabbit.

How should bs-2406R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Aldolase A Polyclonal Antibody, FITC Conjugated (bs-2406R-FITC)?

KLH conjugated synthetic peptide derived from human Aldolase A

What are the excitation and emission wavelengths of the FITC conjugate (bs-2406R-FITC)?

FITC on bs-2406R-FITC: excitation / emission 494nm/518nm.