APNG/MPG Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2412R-Cy5.5-100uL |
| Product Name | APNG/MPG Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APNG/MPG |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4350 |
| Subcellular location | Nucleus |
| Synonyms | N-methylpurine-DNA glycosylase isoform a; DNA 3 methyladenine glycosylase; 3 alkyladenine DNA glycosylase; AAG; ADPG; Alkyladenine DNA glycosylase; anpg ; APNG; MDG; N methylpurine DNA glycosirase; CRA36.1; MDG; Mid1; PIG11; PIG16; 3MG_HUMAN. |
| Background | MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APNG/MPG Polyclonal Antibody, Cy5.5 Conjugated (BS-2412R-CY5-5) validated for, and at what dilution?
BS-2412R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APNG/MPG Polyclonal Antibody, Cy5.5 Conjugated (BS-2412R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig.
How should BS-2412R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APNG/MPG Polyclonal Antibody, Cy5.5 Conjugated (BS-2412R-CY5-5)?
KLH conjugated synthetic peptide derived from human APNG/MPG
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2412R-CY5-5)?
Cy5.5 on BS-2412R-CY5-5: excitation / emission 675nm/694nm.