KIR2DL1 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2419R-Cy3 |
| Product Name | KIR2DL1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD158a/KIR2DL1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3802 |
| Subcellular location | Cell membrane |
| Synonyms | Killer cell immunoglobulin-like receptor 2DL1; CD158A; Killer cell immunoglobulin like receptor 2DL1; MHC class I NK cell receptor; Natural killer associated transcript 1; NKAT 1; NKAT-1; NKAT1; p58 natural killer cell receptor clones CL-42/47.11; p58 NK receptor; p58.1 MHC class I specic NK receptor; CD158 antigen-like family member A; KI2L1_HUMAN; Natural killer-associated transcript 1; p58 NK receptor CL-42/47.11; p58.1 MHC class-I-specic NK receptor; CD158a. |
| Background | The KIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on natural killer (NK) cells and a subset of human T cells. KIR2DL1 is a receptor on human NK cells for HLA-C alleles. It inhibits the activity of NK cells thus preventing cell lysis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is KIR2DL1 Polyclonal Antibody, Cy3 Conjugated (bs-2419R-Cy3) validated for, and at what dilution?
bs-2419R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2419R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise KIR2DL1 Polyclonal Antibody, Cy3 Conjugated (bs-2419R-Cy3)?
KLH conjugated synthetic peptide derived from human CD158a/KIR2DL1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2419R-Cy3)?
Cy3 on bs-2419R-Cy3: excitation / emission 512,550nm/570,615nm.