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DLK1 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-2423R-100uL
Product Name DLK1 Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IF(IHC-P)
Reactivity Human
Predicted Reactivity Mouse, Rat, Sheep, Pig, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse DLK1
Immunogen Range 251-350/383
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8788
Swiss Prot P80370
Subcellular location Cell membrane
Synonyms DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1; Protein delta homolog 1; DLK1
Background This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010].
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is DLK1 Polyclonal Antibody (bs-2423R) validated for, and at what dilution?

bs-2423R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DLK1 Polyclonal Antibody (bs-2423R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Sheep, Pig, Rabbit.

How should bs-2423R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DLK1 Polyclonal Antibody (bs-2423R)?

KLH conjugated synthetic peptide derived from mouse DLK1 Immunogen range: 251-350/383.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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