DLK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2423R-BF594-100uL |
| Product Name | DLK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse DLK1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8788 |
| Swiss Prot | P80370 |
| Subcellular location | Cell membrane |
| Synonyms | DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1; Protein delta homolog 1; DLK1 |
| Background | This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is DLK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-2423R-BF594) validated for, and at what dilution?
bs-2423R-BF594 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DLK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-2423R-BF594) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Sheep, Pig, Rabbit.
How should bs-2423R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DLK1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-2423R-BF594)?
KLH conjugated synthetic peptide derived from mouse DLK1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-2423R-BF594)?
AbBy Fluor-594 on bs-2423R-BF594: excitation / emission 590nm/617nm.