Help

KCNH1 Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2424R-BF405
Product Name KCNH1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human KCNH1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3756
Subcellular location Cell membrane
Synonyms EAG1; EAG 1; EAG-1; KCNH 1; KCNH-1; h eag; Kv10.1; MGC124420; MGC142269; EAG; Ether a go go potassium channel 1; hEAG1; M eag; MGC124419; Potassium voltage gated channel subfamily H eag related, member 1; Voltage gated potassium channel subunit Kv10.1; KCNH1_HUMAN.
Background Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is KCNH1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-2424R-BF405) validated for, and at what dilution?

bs-2424R-BF405 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KCNH1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-2424R-BF405) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.

How should bs-2424R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KCNH1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-2424R-BF405)?

KLH conjugated synthetic peptide derived from human KCNH1

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-2424R-BF405)?

AbBy Fluor-405 on bs-2424R-BF405: excitation / emission 401nm/421nm.