Apelin Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2425R-BF647 |
| Product Name | Apelin Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | The antibody's immunogen sequence is derived from the Apelin-13 peptide. Therefore the Antibody can detect each of the active peptides that can be produced via proteolytic processing of the Apelin precursor. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Apelin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8862 |
| Swiss Prot | Q9ULZ1 |
| Subcellular location | Secreted |
| Synonyms | AGTRL1 ligand; APEL; APEL_HUMAN; Apelin-13; Apelin13; Apelin 13; APJ endogenous ligand; Apln; XNPEP2 |
| Background | Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Apelin Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2425R-BF647) validated for, and at what dilution?
bs-2425R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Apelin Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2425R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-2425R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Apelin Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-2425R-BF647)?
KLH conjugated synthetic peptide derived from human Apelin
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-2425R-BF647)?
AbBy Fluor-647 on bs-2425R-BF647: excitation / emission 650nm/665nm.