Dysferlin Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2429R-Cy5 |
| Product Name | Dysferlin Polyclonal Antibody, Cy5 Conjugated |
| Applications |
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DYSF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8291 |
| Swiss Prot | O75923 |
| Subcellular location | Cell membrane |
| Synonyms | DMAT; DYSF; Dysferlin Dystrophy associated fer 1 like protein Fer 1 like protein 1; Dysferlin limb girdle muscular dystrophy 2B autosomal recessive; Dysferlin limb girdle muscular dystrophy 2B; Dystrophy associated fer 1 like 1; Dystrophy associated fer 1 like protein; Dystrophy associated fer1 like 1; Dystrophy associated fer1 like protein; Fer 1 like protein 1; Fer1 like protein 1; FER1L1; FLJ00175; FLJ90168; LGMD 2B; LGMD2B; Limb girdle muscular dystrophy 2B autosomal recessive ; Limb girdle muscular dystrophy 2B; Miyoshi myopathy; MM; DYSF_HUMAN. |
| Background | The protein encoded by this gene belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. In addition, the protein encoded by this gene binds caveolin-3, a skeletal muscle membrane protein which is important in the formation of caveolae. Specific mutations in this gene have been shown to cause autosomal recessive limb girdle muscular dystrophy type 2B (LGMD2B) as well as Miyoshi myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2008]. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Dysferlin Polyclonal Antibody, Cy5 Conjugated (bs-2429R-Cy5) validated for, and at what dilution?
bs-2429R-Cy5 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Dysferlin Polyclonal Antibody, Cy5 Conjugated (bs-2429R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.
How should bs-2429R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Dysferlin Polyclonal Antibody, Cy5 Conjugated (bs-2429R-Cy5)?
KLH conjugated synthetic peptide derived from human DYSF
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-2429R-Cy5)?
Cy5 on bs-2429R-Cy5: excitation / emission 625,650nm/670nm.