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RNF43 Polyclonal Antibody, APC Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-24331R-APC
Product Name RNF43 Polyclonal Antibody, APC Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RNF43
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 54894
Swiss Prot Q68DV7
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms RNF124; Ring finger protein 43; URCC; RNF43_HUMAN; E3 ubiquitin-protein ligase RNF43; RING finger protein 43.
Background RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is RNF43 Polyclonal Antibody, APC Conjugated (bs-24331R-APC) validated for, and at what dilution?

bs-24331R-APC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RNF43 Polyclonal Antibody, APC Conjugated (bs-24331R-APC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse.

How should bs-24331R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RNF43 Polyclonal Antibody, APC Conjugated (bs-24331R-APC)?

KLH conjugated synthetic peptide derived from human RNF43

What are the excitation and emission wavelengths of the APC conjugate (bs-24331R-APC)?

APC on bs-24331R-APC: excitation / emission 650nm/660nm.