RNF43 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-24331R-RBITC |
| Product Name | RNF43 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RNF43 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54894 |
| Swiss Prot | Q68DV7 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | RNF124; Ring finger protein 43; URCC; RNF43_HUMAN; E3 ubiquitin-protein ligase RNF43; RING finger protein 43. |
| Background | RNF43 is a probable E3 ubiquitin-protein ligase that promotes cell growth and is upregulated in colon cancer. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RNF43 Polyclonal Antibody, RBITC Conjugated (bs-24331R-RBITC) validated for, and at what dilution?
bs-24331R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNF43 Polyclonal Antibody, RBITC Conjugated (bs-24331R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse.
How should bs-24331R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNF43 Polyclonal Antibody, RBITC Conjugated (bs-24331R-RBITC)?
KLH conjugated synthetic peptide derived from human RNF43
What are the excitation and emission wavelengths of the RBITC conjugate (bs-24331R-RBITC)?
RBITC on bs-24331R-RBITC: excitation / emission 540nm/625nm.