TWIST Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2441R-PE |
| Product Name | TWIST Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will detect TWIST1 and TWIST2 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TWIST1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7291; 117581 |
| Swiss Prot | Q15672; Q8WVJ9 |
| Subcellular location | Nucleus |
| Synonyms | TWIST; bHLHa38; Twist-related protein 1; Class A basic helix-loop-helix protein 38; H-twist; TWIST1; bHLHa39; Class A basic helix-loop-helix protein 39; Dermis expressed protein 1; Dermis-expressed protein 1; DERMO 1; Dermo-1; DERMO1; MGC117334; Twist 2; Twist homolog 2 Drosophila; Twist homolog 2; Twist related bHLH protein Dermo1; Twist related protein 2; Twist-related protein 2; Twist2; TWST2_HUMAN. |
| Background | TWIST1 and TWIST2 are basic helix-loop-helix transcriptional repressors that bind to E boxes in gene promoters, acting as master regulators in a variety of biological processes, including organogenesis, osteogenesis, cancer progression and hematopoietic cell development. Both TWIST1 and TWIST2 are found to act as Homodimers or Heterodimers with another bHLH protein. TWIST1 Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses expression of proinflammatory cytokines such as TNFA and IL1B. TWIST2 inhibits transcriptional activation by MYOD1, MYOG, MEF2A, and MEF2C. Twist2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is TWIST Polyclonal Antibody, PE Conjugated (bs-2441R-PE) validated for, and at what dilution?
bs-2441R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TWIST Polyclonal Antibody, PE Conjugated (bs-2441R-PE) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-2441R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TWIST Polyclonal Antibody, PE Conjugated (bs-2441R-PE)?
KLH conjugated synthetic peptide derived from human TWIST1
What are the excitation and emission wavelengths of the PE conjugate (bs-2441R-PE)?
PE on bs-2441R-PE: excitation / emission 496,564nm/578nm.