MyoD1/Myf3 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2442R-PE-100uL |
| Product Name | MyoD1/Myf3 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse MyoD1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 17927 |
| Swiss Prot | P10085 |
| Subcellular location | Nucleus |
| Synonyms | MYF3; MyoD; Myod-1; bHLHc1; AI53393; Myoblast determination protein 1; Myod1 |
| Background | Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is MyoD1/Myf3 Polyclonal Antibody, PE Conjugated (bs-2442R-PE) validated for, and at what dilution?
bs-2442R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MyoD1/Myf3 Polyclonal Antibody, PE Conjugated (bs-2442R-PE) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.
How should bs-2442R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MyoD1/Myf3 Polyclonal Antibody, PE Conjugated (bs-2442R-PE)?
KLH conjugated synthetic peptide derived from mouse MyoD1
What are the excitation and emission wavelengths of the PE conjugate (bs-2442R-PE)?
PE on bs-2442R-PE: excitation / emission 496,564nm/578nm.