Patched Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-24459R-Cy5.5-100uL |
| Product Name | Patched Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Patched |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5727 |
| Swiss Prot | Q13635 |
| Subcellular location | Cell membrane |
| Synonyms | Protein patched homolog 1; PTCH; PTC1; A230106A15Rik; BCNS; FLJ26746; FLJ42602; Holoprosencephaly 7; HPE7; mes; NBCCS; OTTHUMP00000021709; OTTHUMP00000021710; Patched; Patched (Drosophila) homolog; Patched 1; Patched homolog (Drosophila); Patched homolog 1 (Drosophila); Patched homolog 1; Patched protein homolog 1; PTC; PTC1; PTCH; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH protein; PTCH1; PTCH1 protein; PTCH11; Ptch2; ; Patched / PTCH; PTC1_HUMAN. |
| Background | Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Patched Polyclonal Antibody, Cy5.5 Conjugated (BS-24459R-CY5-5) validated for, and at what dilution?
BS-24459R-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Patched Polyclonal Antibody, Cy5.5 Conjugated (BS-24459R-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig.
How should BS-24459R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Patched Polyclonal Antibody, Cy5.5 Conjugated (BS-24459R-CY5-5)?
KLH conjugated synthetic peptide derived from human Patched
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-24459R-CY5-5)?
Cy5.5 on BS-24459R-CY5-5: excitation / emission 675nm/694nm.