PPM1D Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2447R-PerCP-100uL |
| Product Name | PPM1D Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PPM1D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8493 |
| Swiss Prot | O15297 |
| Subcellular location | Nucleus |
| Synonyms | WIP1; PP2C-DELTA; Protein phosphatase 1D; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1; PPM1D |
| Background | Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PPM1D Polyclonal Antibody, PerCP Conjugated (bs-2447R-PerCP) validated for, and at what dilution?
bs-2447R-PerCP is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PPM1D Polyclonal Antibody, PerCP Conjugated (bs-2447R-PerCP) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Chicken.
How should bs-2447R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PPM1D Polyclonal Antibody, PerCP Conjugated (bs-2447R-PerCP)?
KLH conjugated synthetic peptide derived from human PPM1D
What are the excitation and emission wavelengths of the PerCP conjugate (bs-2447R-PerCP)?
PerCP on bs-2447R-PerCP: excitation / emission 482nm/677nm.