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CLEC7A Polyclonal Antibody

Mouse lung lysates probed with Rabbit Anti-Beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Mouse lung lysates probed with Rabbit Anti-Beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with Beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Thymus lysates probed with Beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Beta Glucan Receptor Polyclonal Antibody(bs-2455R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Beta Glucan Receptor Polyclonal Antibody(bs-2455R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with XXX Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with XXX Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates probed with beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lung tissue lysates probed with beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-2455R-100uL
Product Name CLEC7A Polyclonal Antibody
Applications
WB ELISA FCM IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Beta-glucan receptor
Immunogen Range 41-140/247
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 64581
Swiss Prot Q9BXN2
Subcellular location Cell membrane
Synonyms BGR; CD369; CANDF4; DECTIN1; CLECSF12; C-type lectin domain family 7 member A; Beta-glucan receptor; C-type lectin superfamily member 12; Dendritic cell-associated C-type lectin 1; DC-associated C-type lectin 1; Dectin-1; CLEC7A; UNQ539/PRO1082
Background Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Frequently asked questions

Which applications is CLEC7A Polyclonal Antibody (bs-2455R) validated for, and at what dilution?

bs-2455R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CLEC7A Polyclonal Antibody (bs-2455R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-2455R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CLEC7A Polyclonal Antibody (bs-2455R)?

KLH conjugated synthetic peptide derived from human Beta-glucan receptor Immunogen range: 41-140/247.

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