LASS3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LASS3 Polyclonal Antibody, Unconjugated (bs-24624R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LASS3 Polyclonal Antibody, Unconjugated (bs-24624R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LASS3 Polyclonal Antibody, Unconjugated (bs-24624R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LASS3 Polyclonal Antibody, Unconjugated (bs-24624R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Sheep
Pig

Overview
Catalog #	bs-24624R
Product Name	LASS3 Polyclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LASS3
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	204219
Swiss Prot	Q8IU89
Subcellular location	Cytoplasm, Cell membrane
Synonyms	LAG1 longevity assurance homolog 3; CerS3; LAG1 homolog, ceramide synthase 3; LASS 3; MGC 27091; CERS3_HUMAN.
Background	May be involved in sphingolipid synthesis or its regulation
Application Dilution
IHC-P	ELISA1:500-1000
IHC-F	IHC-P1:200-400
IF