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TNFSF14 Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-2462R-BF750-100uL
Product Name TNFSF14 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TNFSF14
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8740
Swiss Prot O43557
Subcellular location Cytoplasm, Secreted, Cell membrane
Synonyms LTg; TR2; CD258; HVEML; LIGHT; Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand; TNFSF14; UNQ391/PRO726
Background Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TNFSF14 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2462R-BF750) validated for, and at what dilution?

bs-2462R-BF750 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TNFSF14 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2462R-BF750) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-2462R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TNFSF14 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-2462R-BF750)?

KLH conjugated synthetic peptide derived from human TNFSF14

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-2462R-BF750)?

AbBy Fluor-750 on bs-2462R-BF750: excitation / emission 749nm/775nm.