Siglec 7 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2466R-100uL |
| Product Name | Siglec 7 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD328/Siglec 7 |
| Immunogen Range | 401-467/467 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 27036 |
| Subcellular location | Cell membrane |
| Synonyms | Sialic acid-binding Ig-like lectin 7; Adhesion inhibitory receptor molecule 1; Adhesion inhibitory receptor molecule 1, siglec-7; AIRM 1; AIRM1; CD328; CD328 antigen; CDw328; D siglec; D siglec precursor; P75; p75/AIRM1; QA79; QA79 membrane protein; Sialic acid binding Ig like lectin 7 precursor; Sialic acid binding immunoglobulin like lectin 7; SIGLEC7; SIGL7_HUMAN. |
| Background | Siglec 7 is a member of the Siglec (sialic acid-binding immunoglobulin like lectin) subgroup of the immunoglobulin superfamily. Siglec 7 is able to mediate high levels of sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates. Addition of anti-Siglec 7 to haematopoietic cell cultures leads to reduced cell growth and prevents the development of dendritic cells. Predominantly expressed by resting and activated natural killer cells and at lower levels by granulocytes and monocytes. High expression can be found in tissues like the placenta, liver, lung, spleen, and peripheral blood leukocytes. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Siglec 7 Polyclonal Antibody (bs-2466R) validated for, and at what dilution?
bs-2466R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2466R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Siglec 7 Polyclonal Antibody (bs-2466R)?
KLH conjugated synthetic peptide derived from human CD328/Siglec 7 Immunogen range: 401-467/467.