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CD30 Polyclonal Antibody

Formalin-fixed and paraffin embedded human kidney tissue labeled with Anti CD30\/TNFRSF8\/CD153 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney tissue labeled with Anti CD30\/TNFRSF8\/CD153 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: A431 cell lysates probed with CD30 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: A431 cell lysates probed with CD30 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD30 Polyclonal Antibody, Unconjugated (bs-2495R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Pig

Predicted Reactivity

  • Rabbit
Overview
Catalog # bs-2495r
Product Name CD30 Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Pig
Predicted Reactivity Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD30
Immunogen Range 131-230/595
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 943
Swiss Prot P28908
Subcellular location Cytoplasm, Cell membrane
Synonyms CD3; Ki-1; D1S166E; Tumor necrosis factor receptor superfamily member 8; CD3L receptor; Ki-1 antigen; Lymphocyte activation antigen CD3; TNFRSF8
Background Receptor for TNFSF8/CD3L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CD30 Polyclonal Antibody (bs-2495R) validated for, and at what dilution?

bs-2495R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD30 Polyclonal Antibody (bs-2495R) react with?

Tested reactivity: Human, Pig. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.

How should bs-2495R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD30 Polyclonal Antibody (bs-2495R)?

KLH conjugated synthetic peptide derived from human CD30 Immunogen range: 131-230/595.

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