CD30 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2495r-percp-cy5.5-100uL |
| Product Name | CD30 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Pig |
| Predicted Reactivity | Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD30 |
| Immunogen Range | 131-230/595 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 943 |
| Swiss Prot | P28908 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CD3; Ki-1; D1S166E; Tumor necrosis factor receptor superfamily member 8; CD3L receptor; Ki-1 antigen; Lymphocyte activation antigen CD3; TNFRSF8 |
| Background | Receptor for TNFSF8/CD3L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD30 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-2495R-PERCP-CY5-5) validated for, and at what dilution?
BS-2495R-PERCP-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD30 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-2495R-PERCP-CY5-5) react with?
Tested reactivity: Human, Pig. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.
How should BS-2495R-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD30 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-2495R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human CD30 Immunogen range: 131-230/595.
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-2495R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-2495R-PERCP-CY5-5: excitation / emission 482nm/694nm.