CD69 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Daudi (H) treated with PMA (50 ng\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CD69 polyclonal antibody, unconjugated (bs-2499R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Daudi (H) treated with PMA (50 ng\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CD69 polyclonal antibody, unconjugated (bs-2499R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Overview
Catalog #	bs-2499R
Product Name	CD69 Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human
Predicted Reactivity	Rat, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD69
Immunogen Range	101-199/199
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	969
Swiss Prot	Q07108
Subcellular location	Cell membrane
Synonyms	AIM; BL-AC/P26; CLEC2C; EA1; GP32/28; MLR-3; 5830438K24Rik; VEA; CD69_HUMAN; CD69; Activation inducer molecule (AIM); C-type lectin domain family 2 member C; Early T-cell activation antigen p60; Leukocyte surface antigen Leu-23; CD69_MOUSE; CD69 molecule; CD69 antigen (p60, early T-cell activation antigen)
Background	Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200