CYP1A2 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-25008R-RBITC |
| Product Name | CYP1A2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Dog |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CYP1A2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13077 |
| Swiss Prot | P00186 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Cytochrome P450 1A2; cytochrome P450, family 1, subfamily a, polypeptide 2; Cytochrome P450 family 1 polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; Cyp1a2; CYPD45; P-450d; RATCYPD45; CP12; CP 12; P3-450; Aryl hydrocarbon hydroxylase; Dioxin inducable P3 45; Flavoprotein linked monooxygenase; Microsomal monooxygenase; P450 4; P450 form 4; P450 P3; P450(PA); Xenobiotic monooxygenase; P450 1A2. |
| Background | Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CYP1A2 Polyclonal Antibody, RBITC Conjugated (bs-25008R-RBITC) validated for, and at what dilution?
bs-25008R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP1A2 Polyclonal Antibody, RBITC Conjugated (bs-25008R-RBITC) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog.
How should bs-25008R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CYP1A2 Polyclonal Antibody, RBITC Conjugated (bs-25008R-RBITC)?
KLH conjugated synthetic peptide derived from mouse CYP1A2
What are the excitation and emission wavelengths of the RBITC conjugate (bs-25008R-RBITC)?
RBITC on bs-25008R-RBITC: excitation / emission 540nm/625nm.