ATG5/APG5L Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-25013R-HRP-100uL |
| Product Name | ATG5/APG5L Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATG5/APG5L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9474 |
| Swiss Prot | Q9H1Y0 |
| Subcellular location | Cytoplasm |
| Synonyms | APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG5_HUMAN. |
| Background | Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:100-500 |
| IHC-F | IHC-F=1:100-500 |
Frequently asked questions
Which applications is ATG5/APG5L Polyclonal Antibody, HRP Conjugated (bs-25013R-HRP) validated for, and at what dilution?
bs-25013R-HRP is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG5/APG5L Polyclonal Antibody, HRP Conjugated (bs-25013R-HRP) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig.
How should bs-25013R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATG5/APG5L Polyclonal Antibody, HRP Conjugated (bs-25013R-HRP)?
KLH conjugated synthetic peptide derived from human ATG5/APG5L