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SPARC Polyclonal Antibody

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-25112r
Product Name SPARC Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPARC
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6678
Swiss Prot P09486
Subcellular location Nucleus, Secreted, Extracellular matrix
Synonyms BM-40; OI17; ON; ONT; SPRC_HUMAN; SPARC; Basement-membrane protein 40 (BM-40); Osteonectin (ON); Secreted protein acidic and rich in cysteine;
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:400-800
IF IF=1:100-500

Frequently asked questions

Which applications is SPARC Polyclonal Antibody (bs-25112R) validated for, and at what dilution?

bs-25112R is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPARC Polyclonal Antibody (bs-25112R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Sheep, Pig, Chicken, Rabbit.

How should bs-25112R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPARC Polyclonal Antibody (bs-25112R)?

KLH conjugated synthetic peptide derived from human SPARC