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SPARC Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-25112r-bf350
Product Name SPARC Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPARC
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6678
Swiss Prot P09486
Subcellular location Nucleus, Secreted, Extracellular matrix
Synonyms BM-40; OI17; ON; ONT; SPRC_HUMAN; SPARC; Basement-membrane protein 40 (BM-40); Osteonectin (ON); Secreted protein acidic and rich in cysteine;
Application Dilution
IF =1:100-500

Frequently asked questions

Which applications is SPARC Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-25112R-BF350) validated for, and at what dilution?

bs-25112R-BF350 is validated for IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPARC Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-25112R-BF350) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Sheep, Pig, Chicken, Rabbit.

How should bs-25112R-BF350 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPARC Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-25112R-BF350)?

KLH conjugated synthetic peptide derived from human SPARC

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-25112R-BF350)?

AbBy Fluor-350 on bs-25112R-BF350: excitation / emission 346nm/442nm.