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SPARC Polyclonal Antibody, PE Conjugated

Applications

  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-25112r-pe
Product Name SPARC Polyclonal Antibody, PE Conjugated
Applications
IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPARC
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6678
Swiss Prot P09486
Subcellular location Nucleus, Secreted, Extracellular matrix
Synonyms BM-40; OI17; ON; ONT; SPRC_HUMAN; SPARC; Basement-membrane protein 40 (BM-40); Osteonectin (ON); Secreted protein acidic and rich in cysteine;
Application Dilution
IF =1:100-500

Frequently asked questions

Which applications is SPARC Polyclonal Antibody, PE Conjugated (bs-25112R-PE) validated for, and at what dilution?

bs-25112R-PE is validated for IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPARC Polyclonal Antibody, PE Conjugated (bs-25112R-PE) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Sheep, Pig, Chicken, Rabbit.

How should bs-25112R-PE be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPARC Polyclonal Antibody, PE Conjugated (bs-25112R-PE)?

KLH conjugated synthetic peptide derived from human SPARC

What are the excitation and emission wavelengths of the PE conjugate (bs-25112R-PE)?

PE on bs-25112R-PE: excitation / emission 496,564nm/578nm.