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GGNBP2 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GGNBP2) Polyclonal Antibody, Unconjugated (bs-25129R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-25129r
Product Name GGNBP2 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GGNBP2
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 79893
Swiss Prot Q9H3C7
Synonyms DIF-3; DIF3; FLJ21230; FLJ22561; Gametogenetin-binding protein 2; GGNB2_HUMAN; Ggnbp2; Laryngeal carcinoma-related protein 1; LCRG1; LZK1; Protein ZNF403; ZFP403; ZNF403.
Background

LCRG1 is a 697 amino acid protein that may be involved in spermatogenesis. LCRG1 interacts with gametogenetin, a protein primarily expressed in testis and ovary. Localized in the cytoplasm, LCRG associates with vesicular structures. LCRG1 is expressed in brain, placenta, lung, liver and kidney, with highest levels found in heart, pancreas and skeletal muscle. LCRG1 is strongly down-regulated in 40% of primary laryngeal carcinoma and in 6 of 10 various cancer cell lines. Phosphorylated on Serine 360, LCRG1 exists as three isoforms produced by alternative splicing events.
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:400-800
IF IF=1:100-500

Frequently asked questions

Which applications is GGNBP2 Polyclonal Antibody (bs-25129R) validated for, and at what dilution?

bs-25129R is validated for IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GGNBP2 Polyclonal Antibody (bs-25129R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-25129R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GGNBP2 Polyclonal Antibody (bs-25129R)?

KLH conjugated synthetic peptide derived from human GGNBP2

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