CCR5 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2514R-FITC |
| Product Name | CCR5 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CCR-5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1234 |
| Swiss Prot | P51681 |
| Subcellular location | Cell membrane |
| Synonyms | CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5; C-C chemokine receptor type 5; C-C CKR-5; CCR5; CHEMR13; HIV-1 fusion coreceptor |
| Background | Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CCR5 Polyclonal Antibody, FITC Conjugated (bs-2514R-FITC) validated for, and at what dilution?
bs-2514R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CCR5 Polyclonal Antibody, FITC Conjugated (bs-2514R-FITC) react with?
Tested reactivity: Human.
How should bs-2514R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CCR5 Polyclonal Antibody, FITC Conjugated (bs-2514R-FITC)?
KLH conjugated synthetic peptide derived from human CCR-5
What are the excitation and emission wavelengths of the FITC conjugate (bs-2514R-FITC)?
FITC on bs-2514R-FITC: excitation / emission 494nm/518nm.