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ZCWCC1 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ZCWCC1) Polyclonal Antibody, Unconjugated (bs-25177R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ZCWCC1) Polyclonal Antibody, Unconjugated (bs-25177R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ZCWCC1) Polyclonal Antibody, Unconjugated (bs-25177R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ZCWCC1) Polyclonal Antibody, Unconjugated (bs-25177R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human K562 cell lysates probed with ZCWCC1 Polyclonal Antibody, Unconjugated (bs-25177R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human K562 cell lysates probed with ZCWCC1 Polyclonal Antibody, Unconjugated (bs-25177R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-25177r
Product Name ZCWCC1 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Human, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ZCWCC1
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 22880
Swiss Prot Q9Y6X9
Subcellular location Cytoplasm, Nucleus
Synonyms AC004542.C22.1.; CW type with coiled coil domain 1; KIAA0852; ZCW3; ZCWCC1; Zinc finger; Zinc finger CW type coiled coil domain protein 1; Zinc finger CW type with coiled coil domain 1; Zing finger CW type 3 zinc finger CW-type coiled-coil domain protein 1; MORC family CW-type zine finger 2; MORC2_HUMAN; MORC2.
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:400-800
IF IF=1:100-500

Frequently asked questions

Which applications is ZCWCC1 Polyclonal Antibody (bs-25177R) validated for, and at what dilution?

bs-25177R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-500); IHC-F (IHC-F=1:400-800); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ZCWCC1 Polyclonal Antibody (bs-25177R) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.

How should bs-25177R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ZCWCC1 Polyclonal Antibody (bs-25177R)?

KLH conjugated synthetic peptide derived from human ZCWCC1

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