CD99 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2523R-BF680 |
| Product Name | CD99 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CD99 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 673094 |
| Swiss Prot | Q8VCN6 |
| Subcellular location | Cell membrane |
| Synonyms | D4; Pilr-l; pilr-1; 1110061M03Rik; 2410026K10Rik; CD99 antigen; Paired immunoglobin-like type 2 receptor-ligand; CD99; Pilrl |
| Background | Involved in T-cell adhesion processes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD99 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2523R-BF680) validated for, and at what dilution?
bs-2523R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2523R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD99 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2523R-BF680)?
KLH conjugated synthetic peptide derived from mouse CD99
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-2523R-BF680)?
AbBy Fluor-680 on bs-2523R-BF680: excitation / emission 679nm/702nm.