CD163/M130 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Pig
  • Others

Predicted Reactivity

  • Horse
Overview
Catalog # bs-2527R-Biotin
Product Name CD163/M130 Polyclonal Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P, IHC-F
Reactivity Human, Mouse, Rat, Dog, Pig, Others
Predicted Reactivity Horse
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD163/M130
Immunogen Range 1001-1121/1156
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 9332
Swiss Prot Q86VB7
Subcellular location Secreted, Cell membrane
Synonyms M13; MM13; Scavenger receptor cysteine-rich type 1 protein M13; Hemoglobin scavenger receptor; CD163, M130
Background Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500