MuRF1 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2539R-BF555 |
| Product Name | MuRF1 Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MuRF1/Trim63 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84676 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MuRF 1; MuRF-1; Muscle-specic RING finger protein 1; Muscle-specic RING finger protein 1; E3 ubiquitin-protein ligase TRIM63; FLJ32380; IRF; MURF1; MURF 1; MURF2; RNF28; SMRZ; Iris ring finger protein; Muscle specic ring finger protein 2; Ring finger protein 28; RNF28; SMRZ; Striated muscle RING zinc finger protein; TRIM 63; TRIM63; Tripartite mot containing 63; Tripartite mot containing protein 63; Ubiquitin ligase TRIM63. |
| Background | This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MuRF1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2539R-BF555) validated for, and at what dilution?
bs-2539R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MuRF1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2539R-BF555) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig, Horse, Rabbit.
How should bs-2539R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MuRF1 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2539R-BF555)?
KLH conjugated synthetic peptide derived from human MuRF1/Trim63
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-2539R-BF555)?
AbBy Fluor-555 on bs-2539R-BF555: excitation / emission 553nm/568nm.