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CLEC1 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • GuineaPig

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-2542R-Cy3
Product Name CLEC1 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, GuineaPig
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CLEC1/CLEC1A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51267
Swiss Prot Q8NC01
Subcellular location Cell membrane
Synonyms CLEC1; CLEC-1; C-type lectin domain family 1 member A; C-type lectin-like receptor 1; CLEC1A; UNQ569/PRO1131
Background This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded protein may play a role in regulating dendritic cell function. Alternative splice variants have been described but their biological nature has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CLEC1 Polyclonal Antibody, Cy3 Conjugated (bs-2542R-Cy3) validated for, and at what dilution?

bs-2542R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CLEC1 Polyclonal Antibody, Cy3 Conjugated (bs-2542R-Cy3) react with?

Tested reactivity: Mouse, GuineaPig. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.

How should bs-2542R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CLEC1 Polyclonal Antibody, Cy3 Conjugated (bs-2542R-Cy3)?

KLH conjugated synthetic peptide derived from human CLEC1/CLEC1A

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2542R-Cy3)?

Cy3 on bs-2542R-Cy3: excitation / emission 512,550nm/570,615nm.