phospho-MLKL (Ser345), Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-25447r-cy3 |
| Product Name | phospho-MLKL (Ser345), Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from mouse MLKL around the phosphorylation site of Ser345 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 197259 |
| Swiss Prot | Q9D2Y4 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | phospho-MLKL(S345); p-MLKL(S345); MLKL(p-S345); hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain-like protein; mixed lineage kinase domain like pseudokinase; MLKL_HUMAN. |
| Application Dilution | |
| WB | 1:200-1000 |
Frequently asked questions
Which applications is phospho-MLKL (Ser345), Cy3 Conjugated (bs-25447R-Cy3) validated for, and at what dilution?
bs-25447R-Cy3 is validated for WB (1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MLKL (Ser345), Cy3 Conjugated (bs-25447R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bs-25447R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MLKL (Ser345), Cy3 Conjugated (bs-25447R-Cy3)?
KLH conjugated Synthesised phosphopeptide derived from mouse MLKL around the phosphorylation site of Ser345
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-25447R-Cy3)?
Cy3 on bs-25447R-Cy3: excitation / emission 512,550nm/570,615nm.