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EphB6 Polyclonal Antibody

Mouse kidney cells probed with Rabbit Anti-EphB6 Polyclonal Antibody (bs-2571R) at 1:50 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Mouse kidney cells probed with Rabbit Anti-EphB6 Polyclonal Antibody (bs-2571R) at 1:50 for 60 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue).
Lane 1: U2os cell lysates probed with EphB6 Polyclonal Antibody, Unconjugated (bs-2571R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: U2os cell lysates probed with EphB6 Polyclonal Antibody, Unconjugated (bs-2571R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA

Reactivity

  • Human
Overview
Catalog # bs-2571R
Product Name EphB6 Polyclonal Antibody
Applications WB, ELISA
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human EphB6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2051
Swiss Prot O15197
Subcellular location Secreted, Cell membrane
Synonyms HEP; Ephrin type-B receptor 6; Tyrosine-protein kinase-defective receptor EPH-6; EPHB6
Background Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000

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