Glycophorin A Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2575R-PE |
| Product Name | Glycophorin A Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Glycophorin A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2993 |
| Swiss Prot | P14220 |
| Subcellular location | Cell membrane |
| Synonyms | Glycophorin A; AI853584; CD235a; GPA; Gypa; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; Glycophorin A MNS blood group; GlycophorinA; GPErik; GpMiIII; GPSAT; GYPA; GYPA, included; HGpMiIII; HgpMiV; HgpMiX; HgpMiXI; HGpStaC; MN; MN sialoglycoprotein; MNS; PAS-2; PAS2. |
| Background | Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors and also binds influenza virus. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Glycophorin A Polyclonal Antibody, PE Conjugated (bs-2575R-PE) validated for, and at what dilution?
bs-2575R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glycophorin A Polyclonal Antibody, PE Conjugated (bs-2575R-PE) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-2575R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Glycophorin A Polyclonal Antibody, PE Conjugated (bs-2575R-PE)?
KLH conjugated synthetic peptide derived from mouse Glycophorin A
What are the excitation and emission wavelengths of the PE conjugate (bs-2575R-PE)?
PE on bs-2575R-PE: excitation / emission 496,564nm/578nm.