NKG5 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human larynx labeled with Rabbit Anti-GNLY\/NKG5 Polyclonal Antibody, Unconjugated (bs-2577R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human larynx labeled with Rabbit Anti-GNLY\/NKG5 Polyclonal Antibody, Unconjugated (bs-2577R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$paraformaldehyde-fixed, paraffin embedded human laryngocarcinoma tissue; Antigen retrieval with 0.01M citrate buffer(pH6) boiling for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-GNLY\/NKG5 Polyclonal Antibody, Unconjugated (bs-2577R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

paraformaldehyde-fixed, paraffin embedded human laryngocarcinoma tissue; Antigen retrieval with 0.01M citrate buffer(pH6) boiling for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-GNLY\/NKG5 Polyclonal Antibody, Unconjugated (bs-2577R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-2577R
Product Name	NKG5 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GNLY/NKG5
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10578
Swiss Prot	P22749
Subcellular location	Secreted
Synonyms	LAG2; NKG5; LAG-2; D2S69E; TLA519; Granulysin; Lymphokine LAG-2; Protein NKG5; T-cell activation protein 519; GNLY
Background	Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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