Granzyme B + D + E + N + G + F + H Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2579R-BF594-100uL |
| Product Name | Granzyme B + D + E + N + G + F + H Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Granzyme D/B/E/N/G/F/H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2999 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane |
| Synonyms | Granzyme D; Granzyme B; Granzyme E; Granzyme N; Granzyme G; Granzyme F; Granzyme H; Cathepsin G-like 2; CCP-X; CCP3; CCP4; CCP5; CGL-2; CSP-C; Ctla-5; Ctla-6;, Ctla-7; CTLA1; CTSGL2; Grz d; Grz e; Grz f; Grz g; GZMD; Gzme; Gzmf; Gzmg; GZMH; MCSP-2; MCSP-3; MGC117682; Rnkp7; mast cell proteinase-3-like; Cytotoxic T-lymphocyte proteinase; Granzyme H precursor; Cytotoxic serine protease-C; CSP-C. |
| Background | protease required for lymphocyte cytotoxic granules to lyse target cells [RGD]. Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Granzyme B + D + E + N + G + F + H Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-2579R-BF594) validated for, and at what dilution?
bs-2579R-BF594 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2579R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Granzyme B + D + E + N + G + F + H Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-2579R-BF594)?
KLH conjugated synthetic peptide derived from human Granzyme D/B/E/N/G/F/H
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-2579R-BF594)?
AbBy Fluor-594 on bs-2579R-BF594: excitation / emission 590nm/617nm.