Granzyme B + D + E + N + G + F + H Polyclonal Antibody, FITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-2579R-FITC-100uL |
| Product Name | Granzyme B + D + E + N + G + F + H Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Granzyme D/B/E/N/G/F/H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2999 |
| Subcellular location | Cytoplasm, Nucleus, Secreted, Cell membrane |
| Synonyms | Granzyme D; Granzyme B; Granzyme E; Granzyme N; Granzyme G; Granzyme F; Granzyme H; Cathepsin G-like 2; CCP-X; CCP3; CCP4; CCP5; CGL-2; CSP-C; Ctla-5; Ctla-6;, Ctla-7; CTLA1; CTSGL2; Grz d; Grz e; Grz f; Grz g; GZMD; Gzme; Gzmf; Gzmg; GZMH; MCSP-2; MCSP-3; MGC117682; Rnkp7; mast cell proteinase-3-like; Cytotoxic T-lymphocyte proteinase; Granzyme H precursor; Cytotoxic serine protease-C; CSP-C. |
| Background | protease required for lymphocyte cytotoxic granules to lyse target cells [RGD]. Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Granzyme B + D + E + N + G + F + H Polyclonal Antibody, FITC Conjugated (bs-2579R-FITC) validated for, and at what dilution?
bs-2579R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-2579R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Granzyme B + D + E + N + G + F + H Polyclonal Antibody, FITC Conjugated (bs-2579R-FITC)?
KLH conjugated synthetic peptide derived from human Granzyme D/B/E/N/G/F/H
What are the excitation and emission wavelengths of the FITC conjugate (bs-2579R-FITC)?
FITC on bs-2579R-FITC: excitation / emission 494nm/518nm.