ICOS Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2583R-Cy3 |
| Product Name | ICOS Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody may also have a secondary cross-reactivity with AP-1 complex-associated regulatory protein due to a 77% non-contiguous sequence similarity in the corresponding immunogen region. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ICOS |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29851 |
| Swiss Prot | Q9Y6W8 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | AILIM; CD278; CVID1; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; ICOS |
| Background | Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ICOS Polyclonal Antibody, Cy3 Conjugated (bs-2583R-Cy3) validated for, and at what dilution?
bs-2583R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ICOS Polyclonal Antibody, Cy3 Conjugated (bs-2583R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Pig.
How should bs-2583R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ICOS Polyclonal Antibody, Cy3 Conjugated (bs-2583R-Cy3)?
KLH conjugated synthetic peptide derived from human ICOS
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2583R-Cy3)?
Cy3 on bs-2583R-Cy3: excitation / emission 512,550nm/570,615nm.