IL5RA Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2601R-Cy5.5-100uL |
| Product Name | IL5RA Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IL-5R alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3568 |
| Swiss Prot | Q01344 |
| Subcellular location | Cell membrane |
| Synonyms | Interleukin-5 receptor alpha; CD125 antigen; CDw125; HSIL5R3; IL5R; IL-5R; Interleukin 5 Receptor alpha; Interleukin 5 receptor subunit alpha; interleukin 5 receptor type 3; MGC26560; IL5RA; IL-5R alpha; IL5RA_HUMAN. |
| Background | The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3(IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5(IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IL5RA Polyclonal Antibody, Cy5.5 Conjugated (BS-2601R-CY5-5) validated for, and at what dilution?
BS-2601R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL5RA Polyclonal Antibody, Cy5.5 Conjugated (BS-2601R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Rabbit.
How should BS-2601R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL5RA Polyclonal Antibody, Cy5.5 Conjugated (BS-2601R-CY5-5)?
KLH conjugated synthetic peptide derived from human IL-5R alpha
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2601R-CY5-5)?
Cy5.5 on BS-2601R-CY5-5: excitation / emission 675nm/694nm.