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IL12RB1 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-2603R-Cy5.5-100uL
Product Name IL12RB1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Rat
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse IL-12RB1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 16161
Swiss Prot Q60837
Subcellular location Extracellular, Cell membrane
Synonyms CD212; IL-12R[b]; Interleukin-12 receptor subunit beta-1; IL-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12R-beta-1; IL-12 receptor beta component; Il12rb1; Il12rb
Background Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IL12RB1 Polyclonal Antibody, Cy5.5 Conjugated (BS-2603R-CY5-5) validated for, and at what dilution?

BS-2603R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IL12RB1 Polyclonal Antibody, Cy5.5 Conjugated (BS-2603R-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should BS-2603R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IL12RB1 Polyclonal Antibody, Cy5.5 Conjugated (BS-2603R-CY5-5)?

KLH conjugated synthetic peptide derived from mouse IL-12RB1

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2603R-CY5-5)?

Cy5.5 on BS-2603R-CY5-5: excitation / emission 675nm/694nm.

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