CD41/ITGA2B Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2636R-PE-Cy5-100uL |
| Product Name | CD41/ITGA2B Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Integrin alpha 2b |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 16399 |
| Swiss Prot | Q9QUM0 |
| Subcellular location | Cell membrane |
| Synonyms | CD41; CD41B; GpIIb; AI172977; alphaIIb; Integrin alpha-IIb; GPalpha IIb; Platelet membrane glycoprotein IIb; Itga2b |
| Background | Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CD41/ITGA2B Polyclonal Antibody, PE-Cy5 Conjugated (bs-2636R-PE-Cy5) validated for, and at what dilution?
bs-2636R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD41/ITGA2B Polyclonal Antibody, PE-Cy5 Conjugated (bs-2636R-PE-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Horse, Rabbit.
How should bs-2636R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD41/ITGA2B Polyclonal Antibody, PE-Cy5 Conjugated (bs-2636R-PE-Cy5)?
KLH conjugated synthetic peptide derived from mouse Integrin alpha 2b
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-2636R-PE-Cy5)?
PE-Cy5 on bs-2636R-PE-Cy5: excitation / emission 488nm/670nm.