ERK1 + ERK2 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2637R-PE |
| Product Name | ERK1 + ERK2 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Goat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse ERK2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26413 |
| Swiss Prot | P63085 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ERK; Erk2; MAPK2; PRKM2; Prkm1; C78273; p41mapk; p42mapk; AA47128; AU18647; 93612K14Rik; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; Mapk1; Mapk; ERK1/2 |
| Background | The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is ERK1 + ERK2 Polyclonal Antibody, PE Conjugated (bs-2637R-PE) validated for, and at what dilution?
bs-2637R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ERK1 + ERK2 Polyclonal Antibody, PE Conjugated (bs-2637R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Goat.
How should bs-2637R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ERK1 + ERK2 Polyclonal Antibody, PE Conjugated (bs-2637R-PE)?
KLH conjugated synthetic peptide derived from mouse ERK2
What are the excitation and emission wavelengths of the PE conjugate (bs-2637R-PE)?
PE on bs-2637R-PE: excitation / emission 496,564nm/578nm.