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CD85c Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-2658R-Cy3
Product Name CD85c Polyclonal Antibody, Cy3 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LIR8/CD85c
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10990
Swiss Prot O75023
Subcellular location Cell membrane
Synonyms LIR8; CD85C; LIR-8; Leukocyte immunoglobulin-like receptor subfamily B member 5; CD85 antigen-like family member C; Leukocyte immunoglobulin-like receptor 8; LILRB5
Background May act as receptor for class I MHC antigens.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CD85c Polyclonal Antibody, Cy3 Conjugated (bs-2658R-Cy3) validated for, and at what dilution?

bs-2658R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD85c Polyclonal Antibody, Cy3 Conjugated (bs-2658R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-2658R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD85c Polyclonal Antibody, Cy3 Conjugated (bs-2658R-Cy3)?

KLH conjugated synthetic peptide derived from human LIR8/CD85c

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2658R-Cy3)?

Cy3 on bs-2658R-Cy3: excitation / emission 512,550nm/570,615nm.