MD1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2661R-Cy3 |
| Product Name | MD1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LY-86 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9450 |
| Subcellular location | Secreted |
| Synonyms | BPAD; dJ80N2.1; LY86; LY-86; LY 86; Lymphocyte antigen 86; Lymphocyte antigen 86 precursor; Major affective disorder 1; MD-1; MD1; MD 1; MD1 protein; MD1 RP105 associated; MD1 RP105 associated; MMD-1; RP1-80N2.1. |
| Background | MD1 is a 28 kDa molecule that is associated at the cell surface with RP105 (CD180). RP105 is a leucine-rich repeat (LRR) molecule that is expressed on B lymphocytes. It was first identified by a mAb that protects spleen B cells from irradiation-induced apoptosis. LRR proteins, such as Toll receptors, have a role in innate immunity. MD1 is primarily expressed by B cells, dendritic cells and monocytes, and promotes the recognition of, and subsequent signaling by LPS in the innate immune system. MD1 does not have any homology to other molecules. MD1 when expressed alone behaves as a secretory protein. Expression of MD1 in a cell increases the expression of RP105. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MD1 Polyclonal Antibody, Cy3 Conjugated (bs-2661R-Cy3) validated for, and at what dilution?
bs-2661R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MD1 Polyclonal Antibody, Cy3 Conjugated (bs-2661R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Rabbit.
How should bs-2661R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MD1 Polyclonal Antibody, Cy3 Conjugated (bs-2661R-Cy3)?
KLH conjugated synthetic peptide derived from human LY-86
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2661R-Cy3)?
Cy3 on bs-2661R-Cy3: excitation / emission 512,550nm/570,615nm.